3OFW
Crystal structure of recombinant Kunitz Type serine protease Inhibitor-1 from the Carribean sea anemone stichodactyla helianthus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-05-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 37.158, 37.158, 114.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.350 - 2.500 |
| R-factor | 0.19118 |
| Rwork | 0.190 |
| R-free | 0.22103 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1shp |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.402 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.358 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 3127 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288.15 | 1.7 M ammonium sulphate, 0.1 M Tris-HCl, 6% glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 288.15K |
