3OFG
Structured Domain of Caenorhabditis elegans BMY-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-24 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.522, 38.148, 40.706 |
| Unit cell angles | 63.39, 85.74, 84.69 |
Refinement procedure
| Resolution | 34.033 - 1.367 |
| R-factor | 0.1467 |
| Rwork | 0.146 |
| R-free | 0.16780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.1) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.370 |
| High resolution limit [Å] | 1.370 |
| Number of reflections | 33444 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 15-18% PEG 3350, 300-500mM LiCl2, 100mM Mes pH 5.5, vapor diffusion, hanging drop, temperature 293K |
