3OEU
Structure of yeast 20S open-gate proteasome with Compound 24
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 136.774, 299.668, 145.301 |
| Unit cell angles | 90.00, 113.22, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.2098 |
| Rwork | 0.209 |
| R-free | 0.24870 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.161 |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 5.620 | 2.600 |
| Rmerge | 0.099 | 0.054 | 0.397 |
| Number of reflections | 324018 | ||
| <I/σ(I)> | 9.7 | ||
| Completeness [%] | 98.7 | 99.3 | 87.5 |
| Redundancy | 3.7 | 3.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MES, pH 7.0, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, vapor diffusion, hanging drop, temperature 293K |
