3OER
Crystal structure of trimeric frataxin from the yeast saccharomyces cerevisiae, complexed with cobalt
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.605 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 121.200, 121.200, 121.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.570 - 3.200 |
| R-factor | 0.20883 |
| Rwork | 0.204 |
| R-free | 0.25550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fql |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.832 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.280 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.046 | 0.493 |
| Number of reflections | 4752 | |
| <I/σ(I)> | 25.38 | 3.1 |
| Completeness [%] | 99.8 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 288 | 1.7 M (NH4)2SO4, 0.2 M Li2SO4, 4 % -butyrolactone, 0.1 M Tris pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
