3OD9
Crystal structure of PliI-Ah, periplasmic lysozyme inhibitor of I-type lysozyme from Aeromonas hydrophyla
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.021, 60.327, 82.187 |
| Unit cell angles | 90.00, 115.65, 90.00 |
Refinement procedure
| Resolution | 46.435 - 1.411 |
| R-factor | 0.1629 |
| Rwork | 0.161 |
| R-free | 0.19720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.075 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.290 |
| High resolution limit [Å] | 1.410 |
| Number of reflections | 79434 |
| Completeness [%] | 91.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.98 | 293 | 1.2M sodium phosphate, 0.8M potassium hydrogen phosphate, 0.1M CAPS, 0.2M LiCl, pH 6.98, vapor diffusion, hanging drop, temperature 293K |
