3OD3
CueO at 1.1 A resolution including residues in previously disordered region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-29 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | .9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.162, 90.766, 53.639 |
| Unit cell angles | 90.00, 102.99, 90.00 |
Refinement procedure
| Resolution | 19.000 - 1.100 |
| R-factor | 0.15542 |
| Rwork | 0.154 |
| R-free | 0.18545 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1kv7 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.420 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | not (necessary) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 1.140 |
| High resolution limit [Å] | 1.100 | 1.100 |
| Rmerge | 0.084 | 0.354 |
| Number of reflections | 186417 | |
| <I/σ(I)> | 8.4 | 2.5 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 5.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 16% POLYETHYLENE GLYCOL 4000, 0.2 M AMMONIUM ACETATE, 0.1 M SODIUM ACETATE, PH 4.6; , VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K , VAPOR DIFFUSION, HANGING DROP |
