3OD0
Crystal structure of cGMP bound cGMP-dependent protein kinase(92-227)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 107.625, 107.625, 171.391 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.708 - 2.900 |
| R-factor | 0.2064 |
| Rwork | 0.204 |
| R-free | 0.25980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.422 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_431)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.103 |
| Number of reflections | 13503 |
| Completeness [%] | 100.0 |
| Redundancy | 29.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277.15 | 0.1M sodium malonate, 12% PEG 3350, 3% D-(+)-glucose monohydrate , pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
