3OCR
Crystal structure of aldolase II superfamily protein from Pseudomonas syringae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 74.407, 74.407, 179.909 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.1858 |
| Rwork | 0.183 |
| R-free | 0.23980 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.285 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.066 | 0.665 |
| Number of reflections | 37674 | |
| <I/σ(I)> | 40.4 | |
| Completeness [%] | 99.2 | 98.6 |
| Redundancy | 11.5 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 297 | PEG4K 30%, Ammonium Acetate 0.2M, tri-Na-Citrate 0.1M, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
