3OC8

Crystal Structure of the C-terminal Domain of the Vibrio cholerae soluble colonization factor TcpF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-08
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	C 2 2 21
Unit cell lengths	64.220, 80.300, 56.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.410 - 2.100
R-factor	0.173
Rwork	0.169
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3oc5
RMSD bond length	0.021
RMSD bond angle	1.824
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.100
Number of reflections	8452
Completeness [%]	96.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	293	1.6 M Ammonium Sulfate, 0.1 M MES pH 6.5, 10% v/v Dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1		6.5	293	1.6 M Ammonium Sulfate, 0.1 M MES pH 6.5, 10% v/v Dioxane, VAPOR DIFFUSION, HANGING DROP, temperature 293K