3OB9
Structure of the human MSL3 chromo-barrel domain at 2.5 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-05-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97934 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 179.178, 36.696, 85.574 |
| Unit cell angles | 90.00, 90.39, 90.00 |
Refinement procedure
| Resolution | 29.400 - 2.500 |
| R-factor | 0.22823 |
| Rwork | 0.226 |
| R-free | 0.27726 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f5k |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.091 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.078 |
| Number of reflections | 19514 |
| <I/σ(I)> | 16.6 |
| Completeness [%] | 99.0 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 2.0 Ammonium Sulfate, 100 mM CHES pH 9.0, , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
