3OAO

Crystal structure of a protein with unknown function from DUF2059 family (PA0856) from PSEUDOMONAS AERUGINOSA at 2.72 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97939,0.91837,0.97895
Spacegroup name	P 32 2 1
Unit cell lengths	72.582, 72.582, 81.498
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.324 - 2.720
R-factor	0.2101
Rwork	0.209
R-free	0.24220
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	0.880
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.324		2.820
High resolution limit [Å]	2.720	5.820	2.720
Rmerge	0.030	0.018	0.943
Number of reflections	6928	1232	1224
<I/σ(I)>	19.96	48.7	1.3
Completeness [%]	98.0	92.2	91.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277	65.0% MPD, 0.1M TRIS pH 8.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K