3OAM
Crystal structure of cytidylyltransferase from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2010-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.928, 68.209, 71.476 |
| Unit cell angles | 82.73, 74.19, 76.97 |
Refinement procedure
| Resolution | 25.060 - 1.750 |
| R-factor | 0.19979 |
| Rwork | 0.198 |
| R-free | 0.23209 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vic |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.228 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.606 | 1.830 |
| High resolution limit [Å] | 1.753 | 1.800 |
| Rmerge | 0.041 | 0.301 |
| Number of reflections | 104474 | |
| <I/σ(I)> | 22.75 | 1.96 |
| Completeness [%] | 94.4 | 92.9 |
| Redundancy | 1.2 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 297 | 1M tri-Na citrate, 0.1M sodium cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
