3O9N
Crystal Structure of a new form of xylanase-A-amylase inhibitor protein(XAIP-III) at 2.4 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-04-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.792, 65.363, 49.418 |
| Unit cell angles | 90.00, 102.13, 90.00 |
Refinement procedure
| Resolution | 19.920 - 2.400 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d5h |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.300 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 10519 | |
| <I/σ(I)> | 8 | 1.7 |
| Completeness [%] | 98.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 0.1M sodium acetate, 20% PEG 6000, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
