3O8Z
Crystal structure of Spn1 (Iws1) core domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-07-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 61.325, 61.325, 116.050 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.645 - 2.150 |
| R-factor | 0.1868 |
| Rwork | 0.185 |
| R-free | 0.22350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.001 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.3) |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.230 |
| High resolution limit [Å] | 2.150 | 4.630 | 2.150 |
| Rmerge | 0.061 | 0.038 | 0.319 |
| Number of reflections | 13913 | ||
| <I/σ(I)> | 19.6 | ||
| Completeness [%] | 99.8 | 99.7 | 98.4 |
| Redundancy | 5.8 | 5.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.01 M magnesium chloride, 0.05 M HEPES pH 7.0, 1.6 M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
