3O8W

Archaeoglobus fulgidus GlnK1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	I 21 3
Unit cell lengths	94.567, 94.567, 94.567
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.900 - 2.280
R-factor	0.17567
Rwork	0.173
R-free	0.22052
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ncp
RMSD bond length	0.023
RMSD bond angle	1.953
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.400
High resolution limit [Å]	2.280
Number of reflections	6605
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	15% PEG 5000 MME, 0.2 M Na-acetate pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K