3O8N

Structure of phosphofructokinase from rabbit skeletal muscle

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-07-07
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.8423
Spacegroup name	P 61 2 2
Unit cell lengths	163.292, 163.292, 356.939
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.200
R-factor	0.2294
Rwork	0.229
R-free	0.30180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o8o
RMSD bond length	0.013
RMSD bond angle	1.862
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.260
High resolution limit [Å]	3.200	3.200
Number of reflections	47202
<I/σ(I)>	16.75	2.6
Completeness [%]	100.0	100
Redundancy	8.3	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	16% PEG 400, 0.1 M MgSO4, 0.1 M acetate buffer pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K