3O81
Beta2-microglobulin from Gallus gallus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-14 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.920, 30.480, 87.360 |
| Unit cell angles | 90.00, 93.47, 90.00 |
Refinement procedure
| Resolution | 36.850 - 2.000 |
| R-factor | 0.19723 |
| Rwork | 0.195 |
| R-free | 0.24189 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bew |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.271 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.850 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 12952 | |
| <I/σ(I)> | 12.5 | 6.8 |
| Completeness [%] | 96.0 | 92.2 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 22%(w/v) PEG 3350, 200mM Mg2Cl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
