3O79
Crystal Structure of Wild-type Rabbit PrP 126-230
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-13 |
Detector | Marmosaic CCD300 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 29.530, 86.170, 87.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.440 - 1.600 |
R-factor | 0.1605 |
Rwork | 0.157 |
R-free | 0.21760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1uw3 |
RMSD bond length | 0.022 |
RMSD bond angle | 1.790 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.440 |
High resolution limit [Å] | 1.600 |
Number of reflections | 30195 |
Completeness [%] | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | hanging drop | 6.5 | 298 | Sodium Cacodylate, NaCl, glycerol, pH 6.5, hanging drop, temperature 298K |