3O74
Crystal structure of Cra transcriptional dual regulator from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.309, 118.506, 61.834 |
| Unit cell angles | 90.00, 107.13, 90.00 |
Refinement procedure
| Resolution | 14.928 - 2.000 |
| R-factor | 0.1856 |
| Rwork | 0.184 |
| R-free | 0.21790 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.710 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 59.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.083 |
| Total number of observations | 254515 |
| Number of reflections | 66131 |
| Completeness [%] | 99.4 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1M Mes pH 6.5, 0.2M NaAc, 15% PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
