3O6N

Crystal Structure of APL1 leucine-rich repeat domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-08-30
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9395
Spacegroup name	P 21 21 21
Unit cell lengths	38.770, 70.350, 161.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.796 - 1.850
R-factor	0.1708
Rwork	0.169
R-free	0.20040
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.791
Data scaling software	XSCALE
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]			1.530
High resolution limit [Å]	1.490	6.670	1.490
Rmerge	0.062	0.013	0.013
Number of reflections	56125	911	1306
<I/σ(I)>	16.72	36.8	0.7
Completeness [%]	76.6	94.3	24.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	16% PEG 8000, 0.1M NaCl, 0.1M Na-Hepes, 0.2M Calcium acetate, pH 7.5, vapor diffusion, temperature 293K, VAPOR DIFFUSION