3O6L
Anti-Tat HIV 11H6H1 Fab' complexed with a 15-mer Tat peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97941 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 123.760, 69.030, 62.530 |
| Unit cell angles | 90.00, 111.40, 90.00 |
Refinement procedure
| Resolution | 19.860 - 2.100 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22723 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mlb |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.047 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.048 | 0.296 |
| Number of reflections | 28645 | |
| <I/σ(I)> | 19.14 | 4.73 |
| Completeness [%] | 99.3 | 99.5 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | soaking | 6.5 | 289 | 0.05M CaCl2, 0.1M Bis-Tris pH 6.5, 30% (v/v) polyethylene glycol 550 monomethyl ether, Soaking of Fab' crystals with 0.1mM Tat-peptide for 1 hour, then 0.5mM for 2 hours, soaking, temperature 289K |
