3O6K
Crystal structure of anti-Tat HIV Fab'11H6H1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-11 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.93950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.143, 69.443, 62.456 |
| Unit cell angles | 90.00, 115.32, 90.00 |
Refinement procedure
| Resolution | 36.930 - 2.000 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mlb |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.999 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.130 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.067 | 0.307 |
| Number of reflections | 32642 | |
| <I/σ(I)> | 12.9 | 4.6 |
| Completeness [%] | 99.5 | 99.7 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.05M CaCl2, 0.1M Bis-Tris pH 6.5, 30% (v/v) polyethylene glycol 550 monomethyl ether , VAPOR DIFFUSION, SITTING DROP, temperature 289K |
