3O60
The Crystal Structure of Lin0861 from Listeria innocua to 2.8A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-09 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 41 |
| Unit cell lengths | 95.457, 95.457, 96.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.690 - 2.800 |
| R-factor | 0.217 |
| Rwork | 0.220 |
| R-free | 0.24600 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.219 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.127 | 0.127 |
| Number of reflections | 531 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 2.5 | 2.5 |
| Redundancy | 1.1 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 297 | 1.26M Ammonium sulfate, 0.1M MES pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
