3O5E
Fk1 domain of FKBP51, crystal form VI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.972 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 54.381, 54.381, 90.155 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.600 |
| R-factor | 0.1893 |
| Rwork | 0.187 |
| R-free | 0.22910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o5p |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.387 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.19) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.096 | 41.743 | 1.690 |
| High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
| Rmerge | 0.086 | 0.054 | 0.409 |
| Total number of observations | 2936 | 13021 | |
| Number of reflections | 20975 | ||
| <I/σ(I)> | 10.8 | 11.1 | 1.8 |
| Completeness [%] | 99.9 | 98.9 | 100 |
| Redundancy | 4.4 | 3.9 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 1.8 M (NH4)3citrate, pH 7.0, vapor diffusion, temperature 293K |
