3O53

Crystal Structure of LRIM1 leucine-rich repeat domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97940
Spacegroup name	P 41 21 2
Unit cell lengths	79.265, 79.265, 238.643
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.667 - 2.000
R-factor	0.1913
Rwork	0.189
R-free	0.23260
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.722
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.030
High resolution limit [Å]	2.000	5.430	2.000
Rmerge	0.067	0.031	0.657
Number of reflections	52399
<I/σ(I)>	11.5
Completeness [%]	99.5	96.7	95.1
Redundancy	7.6	7.1	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	16% PEG 8000, 0.1M NaCl, 0.1M Na-Hepes, 0.2M Calcium acetate, pH 7.5, vapor diffusion, temperature 293K, VAPOR DIFFUSION
1	VAPOR DIFFUSION	7.5	293	16% PEG 8000, 0.1M NaCl, 0.1M Na-Hepes, 0.2M Calcium acetate, pH 7.5, vapor diffusion, temperature 293K, VAPOR DIFFUSION