3O4Q

Crystal structure of the Rous Associated Virus Integrase catalytic domain A182T in citrate buffer pH 6.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979680
Spacegroup name	P 43 21 2
Unit cell lengths	65.490, 65.490, 79.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 1.550
R-factor	0.16646
Rwork	0.164
R-free	0.21125
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.026
RMSD bond angle	1.722
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	REFMAC (5.5.0109)
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.600
High resolution limit [Å]	1.550	1.550
Number of reflections	25642
<I/σ(I)>	20.94	5.36
Completeness [%]	97.4	95.2
Redundancy	4.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	290	20% (w/v) PEG 4000, 10% isopropanol and 0.1 M Na citrate pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 290K