3O35
Crystal structure of TRIM24 PHD-Bromo complexed with H3(23-31)K27ac peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 197 |
Detector technology | CCD |
Collection date | 2009-06-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0809 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.632, 36.498, 128.824 |
Unit cell angles | 90.00, 109.99, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.760 |
Rwork | 0.206 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.240 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.820 |
High resolution limit [Å] | 1.760 | 1.760 |
Number of reflections | 38897 | |
<I/σ(I)> | 28 | 2.3 |
Completeness [%] | 98.7 | 91 |
Redundancy | 5 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 200 mM Na-acetate, 30% Polyethylene glycol 4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |