3O20

Electron transfer complexes:experimental mapping of the Redox-dependent Cytochrome C electrostatic surface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Spacegroup name	C 1 2 1
Unit cell lengths	90.092, 52.029, 77.749
Unit cell angles	90.00, 123.07, 90.00

Refinement procedure

Resolution	65.090 - 1.900
R-factor	0.19712
Rwork	0.193
R-free	0.27616
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hrc
RMSD bond length	0.020
RMSD bond angle	1.794
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	65.090
High resolution limit [Å]	1.900
Number of reflections	21667

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	300	Excess of NaNO3 and 10% of Ditionite, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K