3O1Q

Native Crystal Structure of Helicobacter pylori Urease Accessory Protein UreF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	93
Detector technology	IMAGE PLATE
Collection date	2010-06-10
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	C 1 2 1
Unit cell lengths	135.328, 89.997, 66.950
Unit cell angles	90.00, 94.56, 90.00

Refinement procedure

Resolution	40.225 - 1.850
R-factor	0.1699
Rwork	0.169
R-free	0.20830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.926
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (1.6.1_357)
Refinement software	PHENIX ((phenix.refine: 1.6.1_357))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.950	50.950	1.950
High resolution limit [Å]	1.850	5.850	1.850
Rmerge		0.108	0.334
Number of reflections	68238
<I/σ(I)>		16	3.9
Completeness [%]	100.0	99.7	100
Redundancy		4.7	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	100mM Bis-Tris pH 7.0 and 28% PEG MME 5000 , VAPOR DIFFUSION, HANGING DROP, temperature 289K