3O1B
CRYSTAL STRUCTURE OF DIMERIC KLHXK1 IN CRYSTAL FORM II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-28 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.503, 57.036, 72.811 |
| Unit cell angles | 90.00, 110.34, 90.00 |
Refinement procedure
| Resolution | 29.760 - 2.800 |
| R-factor | 0.18288 |
| Rwork | 0.178 |
| R-free | 0.23755 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o08 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.956 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 11502 | |
| <I/σ(I)> | 9.4 | 2.2 |
| Completeness [%] | 82.7 | 62.4 |
| Redundancy | 2.1 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 1microL reservoir + 1microL protein; reservoir: 22% PEG6000, 0.1M Tris pH 8.0, protein: 10mg/ml KlHxk1, 10mM Tris pH 7.4, 1mM EDTA, 1mM DTT, 0.5mM PMSF , VAPOR DIFFUSION, HANGING DROP, temperature 292K |
