3O12
The crystal structure of a functionally unknown protein from Saccharomyces cerevisiae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 112.665, 112.665, 92.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.604 - 1.500 |
| R-factor | 0.1676 |
| Rwork | 0.167 |
| R-free | 0.18530 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.135 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.692 |
| Number of reflections | 36147 | |
| <I/σ(I)> | 37.9 | 2.7 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 14.5 | 13.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 0.1M sodium citrate, 0.2M K/Na Tartrate, 2M Ammonium sulfate, 1/10 Elastase, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
