3O0V

Crystal structure of the calreticulin lectin domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-04
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9779
Spacegroup name	P 21 21 21
Unit cell lengths	43.111, 75.320, 79.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.790 - 2.300
R-factor	0.21633
Rwork	0.214
R-free	0.27469
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3o0w
RMSD bond length	0.007
RMSD bond angle	0.942
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.790	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	11400
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	295	29% (w/v) PEG 2000 MME, 0.2 M KSCN, 10 mM taurine, and 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K