3O0P
Pilus-related Sortase C of Group B Streptococcus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.087, 37.354, 51.242 |
| Unit cell angles | 90.00, 96.68, 90.00 |
Refinement procedure
| Resolution | 44.781 - 1.300 |
| R-factor | 0.1562 |
| Rwork | 0.155 |
| R-free | 0.18170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w1j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.279 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 40390 | |
| <I/σ(I)> | 38.2 | 3 |
| Completeness [%] | 95.0 | 71.1 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100mM Tris, 200mM NaOAc, 30% PEG-4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
