3O0M
Crystal structure of a ZN-bound histidine triad family protein from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-07 |
| Detector | SATURN |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 59.150, 63.520, 76.500 |
| Unit cell angles | 90.00, 100.51, 90.00 |
Refinement procedure
| Resolution | 35.270 - 1.900 |
| R-factor | 0.159 |
| Rwork | 0.157 |
| R-free | 0.19100 |
| Structure solution method | MR |
| Starting model (for MR) | 1y23 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.362 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.270 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.040 | |
| Number of reflections | 21077 | |
| <I/σ(I)> | 23.01 | 4.9 |
| Completeness [%] | 95.5 | 80.5 |
| Redundancy | 3.5 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | JCSG A4; 30 % MPD, 0.1 M ACETATE PH 4.6, 20 MM CACL2, MYSMA.00754.A.A1 AT 19.9 MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K, temperature 289K |
