3O0E
Crystal structure of OmpF in complex with colicin peptide OBS1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9689 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.491, 101.618, 162.142 |
| Unit cell angles | 90.00, 94.46, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.010 |
| R-factor | 0.2669 |
| Rwork | 0.265 |
| R-free | 0.30250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1opf |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.519 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.120 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.078 | 0.232 |
| Number of reflections | 47846 | 65923 |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 72.9 | 17.1 |
| Redundancy | 2.2 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25% PEG 3350, 0.2M Li2SO4, 0.1M sodium cacodylate, pH 6.5, sitting drop vapor diffusion, temperature 293K |
