3O07
Crystal structure of yeast pyridoxal 5-phosphate synthase Snz1 complexed with substrate G3P
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-25 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 1.0 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 60.474, 111.972, 158.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.200 - 1.800 |
R-factor | 0.20505 |
Rwork | 0.203 |
R-free | 0.23583 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o05 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.167 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 95196 | |
Completeness [%] | 99.5 | 99.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% PEG 400, 0.1M Megnesium Chloride, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |