3O05
Crystal Structure of Yeast Pyridoxal 5-Phosphate Synthase Snz1 Complxed with Substrate PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2009-07-15 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 58.972, 109.421, 155.389 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.400 - 2.200 |
| R-factor | 0.19563 |
| Rwork | 0.194 |
| R-free | 0.22740 |
| Structure solution method | MIR |
| Starting model (for MR) | 1znn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.095 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.400 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 51330 | |
| Completeness [%] | 99.2 | 97.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 20% PEG 400, 0.1M Megnesium Cloride, 0.1M HEPES, 2.5mM PLP, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
