3NY7
STAS domain of YchM bound to ACP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 105 |
Detector technology | IMAGE PLATE |
Collection date | 2009-02-28 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 33.631, 55.867, 113.411 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.797 - 1.922 |
R-factor | 0.165 |
Rwork | 0.162 |
R-free | 0.21900 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.015 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.990 |
High resolution limit [Å] | 1.920 | 1.920 |
Rmerge | 0.338 | |
Number of reflections | 16742 | |
<I/σ(I)> | 3.8 | |
Completeness [%] | 98.0 | 81.8 |
Redundancy | 5.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 298 | 20% PEG 8000, 10 mM Na Citrate , pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 298K |