3NXH
Crystal Structure of the transcriptional regulator yvhJ from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR735.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97903 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.136, 67.800, 85.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.266 - 2.584 |
| R-factor | 0.247 |
| Rwork | 0.245 |
| R-free | 0.29630 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.412 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.584 | 2.600 |
| Rmerge | 0.050 | 0.297 |
| Number of reflections | 14808 | |
| <I/σ(I)> | 42.6 | 6.9 |
| Completeness [%] | 98.1 | 100 |
| Redundancy | 7.4 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under Paraffin oil | 291 | 20% PEG 1000, 0.1M ammonium chloride, 0.1M sodium acetate, Microbatch under Paraffin oil, temperature 291K |
