3NWV
Human cytochrome c G41S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-05-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.632, 62.693, 35.470 |
| Unit cell angles | 90.00, 89.78, 90.00 |
Refinement procedure
| Resolution | 19.790 - 1.900 |
| R-factor | 0.17598 |
| Rwork | 0.172 |
| R-free | 0.24717 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.861 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.790 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.098 | 0.240 |
| Number of reflections | 30928 | |
| <I/σ(I)> | 6.7 | 2.9 |
| Completeness [%] | 96.2 | 93.6 |
| Redundancy | 3.13 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 30% (w/v) PEG 1000, 45mM KH2PO4, 5mM dithiothrietol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
