3NWT

Crystal structure of the N-terminal domain of the yeast telomere-binding and telomerase regulatory protein Cdc13

Experimental procedure

Experimental method	SIRAS
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-08-10
Detector	ADSC QUANTUM 210
Wavelength(s)	0.978
Spacegroup name	P 21 21 2
Unit cell lengths	61.990, 70.200, 53.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.23071
Rwork	0.229
R-free	0.27185
Structure solution method	SIRAS
RMSD bond length	0.009
RMSD bond angle	1.279
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.850
High resolution limit [Å]	2.700	2.700
Number of reflections	6168
<I/σ(I)>	14.5	2.5
Completeness [%]	96.5	98.1
Redundancy	4	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291	0.2 M potassium chloride, 0.1 M magnesium acetate tetrahydrate, 0.05 M sodium cacodylate trihydrate, pH 6.5, 10% w/v polyethylene glycol 8,000, VAPOR DIFFUSION, SITTING DROP, temperature 291K