Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-10-26 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 63 |
| Unit cell lengths | 90.443, 90.443, 76.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.173 - 2.000 |
| Rwork | 0.224 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1goj |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.414 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (refmac_5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 4.310 | 2.000 |
| Rmerge | 0.059 | 0.040 | 0.411 |
| Number of reflections | 46976 | 4754 | 4745 |
| <I/σ(I)> | 18.2 | ||
| Completeness [%] | 99.1 | ||
| Redundancy | 5.2 | 5.4 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 1.5M ammonium sulfate, 0.1M TRIS, pH 8.5, vapor diffusion, sitting drop, temperature 291K |
