3NVN
Molecular mechanism of guidance cue recognition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-02-06 |
Detector | MAR scanner 300 mm plate |
Wavelength(s) | 0.97856 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 95.749, 133.494, 172.231 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.530 - 2.260 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.27600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.088 | 0.293 |
Number of reflections | 73704 | |
<I/σ(I)> | 14.89 | 2.97 |
Completeness [%] | 95.5 | |
Redundancy | 8.4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 6% PEG8000, 0.1M HEPES pH7.0, 0.2M NaCl, 0.1M CaCl2, VAPOR DIFFUSION, SITTING DROP, temperature 295K |