3NV9
Crystal structure of Entamoeba histolytica Malic Enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 117.731, 117.731, 157.615 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.160 - 2.250 |
| R-factor | 0.18253 |
| Rwork | 0.179 |
| R-free | 0.24463 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.834 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0095) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.320 | 47.160 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.068 | |
| Number of reflections | 53238 | |
| <I/σ(I)> | 32.3 | |
| Redundancy | 26.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 1.5M Ammonium Sulfate, sodium acetate pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
