3NUU
phosphoinositide-dependent kinase-1 (PDK1) with fragment11
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 122.615, 122.615, 47.328 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.464 - 1.980 |
| Rwork | 0.189 |
| R-free | 0.23870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.560 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.050 |
| High resolution limit [Å] | 1.980 | 4.260 | 1.980 |
| Rmerge | 0.099 | 0.041 | 0.473 |
| Number of reflections | 25044 | ||
| <I/σ(I)> | 8.7 | ||
| Completeness [%] | 87.2 | 99.8 | 42.8 |
| Redundancy | 8.8 | 10.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 9 | 298 | 2M Ammonium sulfate, 0.1M TRIS, pH 9, sitting drop, temperature 298K |
