3NUS
phosphoinositide-dependent kinase-1 (PDK1) with fragment8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 123.497, 123.497, 47.087 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.095 - 2.750 |
| R-factor | 0.1771 |
| Rwork | 0.174 |
| R-free | 0.23580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.871 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 5.920 | 2.750 |
| Rmerge | 0.114 | 0.051 | 0.477 |
| Number of reflections | 10960 | ||
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 7.4 | 7 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 9 | 298 | 2M Ammonium sulfate, 0.1M TRIS, pH 9, sitting drop, temperature 298K |
