3NTM
Crystal Structure of Tyrosinase from Bacillus megaterium crystallized in the absence of zinc, partial occupancy of CuB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Detector technology | CCD |
| Collection date | 2009-11-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.010, 78.480, 74.550 |
| Unit cell angles | 90.00, 101.61, 90.00 |
Refinement procedure
| Resolution | 39.083 - 2.300 |
| R-factor | 0.1981 |
| Rwork | 0.193 |
| R-free | 0.28870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nm8 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.025 | 39.240 | 2.420 |
| High resolution limit [Å] | 2.300 | 7.270 | 2.300 |
| Rmerge | 0.061 | 0.033 | 0.187 |
| Number of reflections | 22077 | ||
| <I/σ(I)> | 10.5 | 17.8 | 3.8 |
| Completeness [%] | 95.5 | 92.3 | 94 |
| Redundancy | 2.1 | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, sodium cacodylate, pH 6.5, vapor diffusion, hanging drop, temperature 293K |
