3NTL
Crystal Structure of Glucose-1-phosphatase (AgpE) from Enterobacter cloacae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Collection date | 2008-11-04 |
| Wavelength(s) | 0.9184 |
| Spacegroup name | H 3 |
| Unit cell lengths | 151.079, 151.079, 86.644 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.360 - 1.880 |
| R-factor | 0.18466 |
| Rwork | 0.181 |
| R-free | 0.25049 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nt4 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.219 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.053 | 0.721 |
| Number of reflections | 110644 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 91.4 | 45.5 |
| Redundancy | 1.8 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
