3NTJ
Redox regulation of Plasmodium falciparum ornithine delta-aminotransferase
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Wavelength(s) | 0.979335 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 101.390, 104.970, 181.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.825 - 3.000 |
| R-factor | 0.1944 |
| Rwork | 0.191 |
| R-free | 0.24230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1z7d |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.135 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.137 | 0.525 |
| Number of reflections | 39278 | |
| <I/σ(I)> | 14.4 | 3.6 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 2.2 M amonium sulfate, 100mM citriacidacid, 200m MKCL. Protein concentration in the drop 7.5 mg/ml., pH 8.0, VAPOR DIFFUSION, HANGING DROP |
