3NT8
Crystal Structure of Na-ASP-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.780, 74.570, 84.740 |
| Unit cell angles | 90.00, 112.10, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.200 |
| R-factor | 0.19568 |
| Rwork | 0.193 |
| R-free | 0.23747 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u53 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.578 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.800 | 2.300 |
| High resolution limit [Å] | 2.150 | 2.200 |
| Rmerge | 0.107 | 0.557 |
| Number of reflections | 37195 | |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 93.6 | 91.4 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 3UL (7.5 MG/ML) PROTEIN AND 1.5UL RESERVOIR SOLUTION CONTAINING 21% (W/V) PEG 6000 AND 200 MM, SODIUM CITRATE PH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
